Abstract
This study investigates the growth behavior of G. lucidum and P. ostreatus liquid spawn on non-woven hemp mats. A
Complete Yeast Medium (CYM) and Yeast Peptone Dextrose (YPD) medium were used under static (ST) and shaking
(SH) conditions to develop four precultures per species. Mycelium was cultured from agar plates into liquid media
and incubated at 27 °C for 5 days.
Non-woven hemp mats were sterilized and inoculated with 4 dilutions of each precultures. Each mat received 3 mL
of liquid spawn, with biological and technical triplicates. Inoculated mats were incubated at 27 °C for 14 days and
then dried at 60 °C. Daily photographs tracked growth. Using ImageJ, the surface colonization rate was quantified
by calculating the mycelium-covered area as a percentage of the mat. This enabled comparison of media, fungal
species, and dilution effects on growth kinetics